Henrik Y. Rasmussen1, Simone KØ. Sørensen1, Stefan AG. Jahn1, Morten Dahl1,2

Affiliates

1 Dept. Clinical Biochemistry, Zealand University Hospital Køge, Denmark 2 Dept Clinical Medicine, University of Copenhagen, Denmark

Abstract

Background: Reliable measurement of fecal calprotectin is important for the evaluation of gastrointestinal inflammation in patients.
Methods: We compared two different methods for fecal calprotectin measurement: CALiaGold® (Sentinel) and EliATM (Phadia). Over a period of six days 224 routine fecal samples were analyzed on SENTiFIT®270 and ImmunoCap 250 equipment. Three fecal samples were extracted and assayed ten times each to assess the within-run precision by coefficient of variance (CV) percentages.
Results: the comparison between the two methods showed correlation coefficients of 0.90 (y=0.65x + 72.5) in the calprotectin range of 22 to 2000 mg/kg, 0.84 (y=0.71x + 34.0) in the calprotectin range of 22-500 mg/kg, and 0.77 (y=0.90x + 13.9) in the calprotectin range of 22–250 mg/kg. The three within-run precision experiments of the extraction method for CALiaGold® showed CV values of 14%, 9.1% and 7.5%, respectively (maximum acceptable CV was 20%). Corresponding values for EliATM were 3.6%, 5.1% and 5.1%. The three within-run precision experiments for the CALiaGold® assay showed CV values of 4.4%, 1.6% and 2.1% (maximum acceptable CV was 15%). Corresponding values for EliATM were 3.3%, 2.5% and 7.3%.
Conclusions: Our comparison showed an overall acceptable correlation between the two methods, and the two methods’ extraction and assay techniques performed within acceptable limits.